Cloning biologically active geminivirus DNA using PCR and overlapping primers
نویسندگان
چکیده
منابع مشابه
Cloning biologically active geminivirus DNA using PCR and overlapping primers.
We report a new strategy for cloning DNA of the plant-infecting geminiviruses that is based on PCR amplification with primers overlapping the sequence of a restriction site in the viral genome. This method reduces the time and work necessary to obtain biologically active geminivirus DNAs and could be used for other DNA viruses having circular genomes, such as the animalinfecting circoviruses. G...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1993
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/21.5.1325